The researchers have developed a clever new method that can be used to detect single-molecule interactions between receptors and ligands. The researchers point out that this method can be widely used in stem cell, cancer, infectious disease and immunology research And other fields.
The cells in the human body are not the lone men who perform tasks alone, they need to communicate with other cells or substrates. Many functions of cells are activated by signals in the environment, including cell proliferation, cell adhesion, differentiation of stem cells into different cell types, and migration of white blood cells to fight infection. This is a two-way communication at the molecular level. Cells both receive and release signals.
When a specific molecule binds to a receptor that penetrates the cell membrane, it activates the receptor and triggers a series of cascade reactions. Scientists have conducted extensive research on this mechanism. Now, researchers at the University of Illinois have measured for the first time the molecular force required to transmit functional regulatory signals into cells.
The researchers used the DNA strand as a rope tethered to a ligand molecule and used such ligands to bind to integrin. In the TGT system, only when the DNA strand is not broken can cell adhesion be activated. They used the structural characteristics of the DNA double helix to adjust the DNA rope and obtained a series of DNA strands that can withstand different levels of tension.
A new method called TGT (tension gauge tether). In this way, one can analyze the single-molecule interaction when the ligand activates the cell receptor to determine the molecular force required for the above action. The researchers used TGT technology to study the integrin integrin, which is a receptor protein located on the cell membrane. When the ligand molecule binds to it, integrin activates and mediates cell adhesion.
"It's like fishing. If a fish can pull a 30-pound line but keep pulling a 40-pound line, you can judge that its power is between 3040 pounds," Wang explained. "We apply this strategy to the detection of molecular tension. In mammalian cells, ligands require a certain force to activate the membrane protein integrin. We use DNA ropes that can withstand different tensions to fix ligand molecules on a surface When integrin binds to the ligand, the rope will be pulled. We can determine whether the ligand activates the receptor by observing the cell's adhesion state and determine the force on the DNA rope when activated. "
Single-molecule interactions are difficult to monitor, so researchers need to observe receptor behavior by observing cell behavior. Studies have shown that ligands activate the receptor integrin and require approximately 40 pN of force.
Diversification of working postures is very important to your health. Sitting and standing alternate posture office work is becoming more and more popular, and height adjuatble table has also become very popular.
The ergonomic desk makes you no longer sit in the same position for several hours. This diverse office posture can also increase creativity, energy, concentration, and even increase calorie burn.
The Electric Standing Desk has a control panel with 4 memory keys allowing users to save the settings of 4 preset heights
Adjustable Computer Desk,Office Standing Adjustable Desk,Adjustable Height Workbench,Adjustable Height Office Standing Desks
CHEX Electric Standing Desk , https://www.qxhdesk.com