A large number of microorganisms live in the soil, including bacteria, fungi, actinomycetes, viruses and protists. It is estimated that there are 4,000 to 7,000 species of bacteria in 1 g of soil, and the number is nearly 1 billion. However, the soil environment is relatively complex and is rich in interfering substances such as humic acid, which makes extraction of nucleic acids and proteins difficult.
To this end, MO BIO Laboratories recently launched NoviPure? Soil Protein Extraction Kit, which is the first kit on the market that can extract proteins from various soil types. As a specialist in the processing of soil, feces and other complex samples, MO BIO has been focusing on this special field for 20 years. Previously, Biocom introduced the products of MO BIO to extract DNA from complex samples such as soil.
The main components of NoviPure? Soil Protein Extraction Kit are two patented buffers SP1 and SP2. First, a mixture of glass beads and ceramic beads is used to efficiently lyse cells, while solubilizing intracellular and extracellular proteins. This technique avoids the simultaneous purification of interfering substances (such as humic acid), resulting in protein precipitation. This pellet can be resuspended in any buffer for storage or downstream analysis. Compared with traditional methods, protein has fewer impurities and higher yields.
According to reports, the protein extracted by this kit has been successfully used in 1D and 2D gel electrophoresis and mass spectrometry (2D LC-MS / MS). To protect precious samples, all reagents and consumables in the kit are free of proteases.
For researchers studying the content of microorganisms in the soil and determining specific activities, reliable extraction of soil proteins is important. The isolation and identification of proteins is necessary to determine the function of cells, which enables the study of the association between microorganisms and specific functions.
In addition to protein extraction kits, MO BIO also provides a series of soil DNA and RNA extraction kits. Its Power series utilizes patented inhibitor removal technology (IRT) to remove humic acid and brown matter from even the most difficult soil types, helping the success of downstream PCR analysis. Subsequent PCR analysis is suitable for the detection of various organisms, including Gram-positive and Gram-negative bacteria, fungi, algae, actinomycetes, and nematodes.
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