General operation method of solid phase extraction column

General operation of solid phase extraction

Reverse extraction column operation

Normal phase extraction column operation

Ion exchange extraction column

Extracted from polar matrix

Non-polar samples

Extracted from non-polar matrix

Polar samples

Extract charged samples

activation

Rinse the extraction column with 3-5 ml of methanol;

Rinse the extraction column with 3-5 ml of deionized water (or buffer) and keep the extraction column moist

3-5ml non-polar solvent

Elution extraction column

Rinse the extraction column with 3-5 ml of deionized water and / or low ionic strength buffer

Loading

Sample at the appropriate flow rate

Evenly through the extraction column

With a low flow rate, the sample is evenly passed through the extraction column

Cleaning

An extraction column that elutes the adsorbed sample with 5 ml of a solvent of appropriate polarity (commonly used are pure water, buffer, water / organic solvent mixture)

Elute the extraction column with 5 ml of a suitable non-polar solvent

Rinse the extraction column with 5 ml of deionized water and / or low ionic strength buffer

Elution

The test sample is eluted in batches with 1-5 ml non-polar solvent to the collection container

The test sample is eluted in batches with 1-5 ml of polar solvent to the collection container

The test sample is eluted with 1-5 ml of high ionic strength buffer to the collection container