GAPDH Antibody Daquan-there is always a suitable GAPDH antibody
In the Western Bloting experiment, there may be inaccurate measurement of the total protein concentration, or operation errors between samples generated when the protein sample is loaded before electrophoresis; these errors can be determined by measuring the internal reference actually transferred to the membrane in each sample, such as The content of internal reference GAPDH was used for correction. Of course, in addition to GAPDH antibodies, β-Actin antibodies, β-Tubulin antibodies, etc. are also the most common internal reference antibodies. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme involved in glycolysis. It consists of four 30-40kDa subunits with a molecular weight of 146kDa and a detection band of approximately 36kDa. The GAPDH gene is expressed at a high level in almost all tissues and is widely used as an internal reference for Western blot protein standardization. 
It is particularly important to select the appropriate GAPDH antibody. First, the WB detection band of GAPDH is about 36kDa, so it is particularly suitable for some target proteins with molecular weights greater than 40kDa or less than 30kDa. Generally speaking, domestic antibodies have insufficient quality stability and potency, but the prices of imported brands such as Abcam, Millipore, CST, etc. are relatively high, generally around 3,000 / 100ul. Below we recommend three GAPDH imported antibodies for you, which are the products of American EarthOx and ProteinTech. EarthOx's Anti-GAPDH Monoclonal Antibody (Cat. No. E021010) is a mouse-derived monoclonal antibody, and its titer is very high. The manufacturer's recommended WB initial dilution ratio is 1: 1000. In practical applications, 1: 10000 or more is used. A high report, much higher than this dilution ratio, indicates that its immunopotency is very high. In addition, there are many species tested, including humans, cattle, pigs, mice, rats, etc. The picture on the right is the WB detection results of EarthOx Anti-GAPDH Monoclonal Antibody (Cat. No. E021010) in different cell lines.
ProteinTech's GAPDH Polyclonal Antibody (Cat. No. 10494-1-AP) is a rabbit-derived polyclonal GAPDH antibody. It is a good choice for customers who require rabbit-derived GAPDH antibody. Similarly, ProteinTech's GAPDH Monoclonal Antibody (Cat. No. 60004-1-Ig) is another monoclonal antibody. In addition to WB and IF, it can also be used in immunohistochemical IHC and other experiments. It should be noted that IF is also one of the commonly used application types of GAPDH antibodies. The picture on the left shows the application of ProteinTech's GAPDH Monoclonal Antibody (Cat. No. 60004-1-Ig) antibody on paraffin-embedded, formalin-fixed sections.
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