Adopt high-performance liquid chromatography, the column is MerckSuper-spher100RP-18; the mobile phase is acetonitrile-0.001mol / L potassium dihydrogen phosphate solution (50:50) (pH 2.0 adjusted with phosphoric acid); the flow rate is 1.2ml / min; The detection wavelength is 205 nm; the column temperature is 40 ° C. The related substances cholic acid, 7-ketocholic acid, chenodeoxycholic acid and cholelithic acid in ursodeoxycholic acid capsules eluted and completely separated within 25 minutes. The bile acid has a good linear relationship between 0.1389-0.2777mg / ml, r = 1.0000, and it can still be detected when the injection concentration is 0.01389mg / ml; the linear relationship between 7-ketocholic acid 0.1067-0.2135mg / ml, r = 1.000. VertexC18 column, mobile phase: methanol / 30mMNaH2PO4 solution / triethylamine = 65/30/1 (adjust the pH to 4.4 with phosphoric acid after mixing the three reagents), temperature: room temperature 22 ℃, flow rate 1.0ml / min, UV2000 UV The wavelength of the detector is 210nm, and the instrument uses STI2000 high-performance liquid chromatograph.
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