The changes of ascorbic acid in rat corpus luteum cells were detected by non-invasive micro-testing technology. John R. Pepperell et al. Proposed the hypothesis that flavonol mobilizes intracellular calcium, and the increase in intracellular calcium ion concentration leads to the generation of reactive oxygen species and the release of ascorbic acid from the luteal cells. They applied fluorescence technology to detect changes in intracellular calcium ions and reactive oxygen species, while using ascorbic acid selective microelectrodes to detect changes in ascorbic acid outside single cells to verify this hypothesis (Control of Ascorbic Acid Efflux in Rat Luteal Cells: Role of Intracellar Calcium and Oxygen Radicals. Am J Physiol Cell Physiol (April 30, 2003). 10.1152 / ajpcell.00587.2002). The authors used fluorescence technology and non-damage micro-measurement technology to detect the changes of calcium ions, reactive oxygen species and ascorbic acid in and out of the cells, and fully verified that when the intracellular calcium and reactive oxygen species changed under the stimulation of yellow alcohol At the same time, the hypothesis that extracellular ascorbic acid also changes accordingly.
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