Experimental principle: This kit uses the double antibody sandwich method to determine the level of human somatostatin in the specimen. Microporous plates were coated with purified human somatostatin antibody to make solid-phase antibodies, and human somatostatin was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled somatostatin ( somatostatin) antibody binding to form antibody-antigen-enzyme-labeled antibody complex, after thorough washing, add substrate TMB for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with somatostatin in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human somatostatin in the sample was calculated by a standard curve.
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