How to write the PEA plant efficiency analyzer part in materials and methods
The leaves were dark acclimated for 30 min, and then the rapid chlorophyll fluorescence induction kinetic curve (OJIP curve) was measured using PEA (Hansatech, UK). The OJIP curve was induced by 3000 μmol · m-2 · s-1 pulse light, and the fluorescence signal record was Starting from 10μs and ending at 1s, the initial rate of recording is 105 kinds of data per second. The OJIP fluorescence induction curve is analyzed by JIP-test (Strasser et al, 1995). The meaning of each parameter and the calculation formula are as follows:
The following parameters are modified according to the parameters used in your experiment.
Photochemical efficiency: TRO / ABS, variable fluorescence at point J: VJ, initial slope of OJIP fluorescence induction curve: Mo, light energy absorbed per unit: ABS / CS, number of reaction centers required to absorb a light quantum: RC / ABS The fluorescence intensity at points O, K, and J of the OJIP fluorescence induction curve are: Fo, FK, and FJ, respectively.
Variable fluorescence at point J VJ = (FJ-Fo) / (Fm-Fo);
PSⅡ photochemical efficiency: φPo≡TRO / ABS;
The probability of trapped excitons transferring electrons to other electron acceptors in the electron transfer chain that exceeds QA: ψO≡ (1-VJ);
Quantum yield for electron transfer: φEo≡ φPo • ψO;
The number of active PSⅡ reaction centers per unit area: RC / CSo = φPo • (VJ / MO) • (ABS / CS);
Photosynthetic performance index based on absorbed light energy: PIABS = (RC / ABS) • (φPo / (1-φPo)) • (ψO / (1-ψO));
Relative variable fluorescence at point K: Wk = (Fk-FO) / (FJ-FO).
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